Comparative Analysis of Local Application of Titanium-Platelet Rich Fibrin (T-PRF) and Leukocyte-Platelet Rich Fibrin (L-PRF) in Bone Defect Healing: A Micro-CT and Histopathological Study in Rabbit Models.

Abstract

This study aims to compare the efficacy of locally applied Titanium-Platelet Rich Fibrin (T-PRF) and Leukocyte-Platelet Rich Fibrin (L-PRF) in bone defect healing using micro-CT analysis and histopathological examinations in rabbit models. Eight healthy male New Zealand rabbits, aged 4-6&#x2009;months, were utilized. Circular bicortical defects (6&#x2009;mm diameter) were created at three areas in each rabbit. One of the defects was chosen as the control group, which received saline solution, while T-PRF and L-PRF were applied to the defect sites in separate experimental groups. Bone regeneration was analyzed using high-resolution micro-CT and histological evaluation post-sacrifice. Statistical analysis assessed the significance of observed differences. Micro-CT analysis revealed significant differences among the groups in bone volume, trabecular thickness, trabecular number, connectivity, and connectivity density (p&#x2009;<&#x2009;0.05). Both T-PRF and L-PRF groups exhibited superior bone parameters compared to controls, with L-PRF showing higher values. Trabecular separation and bone surface area to bone volume ratio did not differ significantly among the groups (p&#x2009;>&#x2009;0.05). Histological examination indicated advanced healing stages in the L-PRF group, suggesting both T-PRF and L-PRF contribute to bone regeneration, with L-PRF demonstrating better effects. The study concludes that while both T-PRF and L-PRF aid in bone regeneration, L-PRF shows superior efficacy. L-PRF is recommended for bone defect healing due to its better performance in enhancing bone parameters.