Comparative analysis of the gut microbiota of grass carp fed with chicken faeces.

Abstract

The gut microbiota is closely related to health and disease. Grass carp (Ctenopharyngodon idella) is an important food fish in China. We aimed to investigate the effect of a chicken faeces diet on the gut microbiota composition of grass carp reared in an integrated farming system in China. Gut microbiota compositions of grass carps fed chicken faeces, a commercial diet, and grass were compared based on 16S rRNA gene sequencing. The major intestinal phyla in grass carps fed chicken faeces were Firmicutes, Proteobacteria, and Actinobacteria. The untreated chicken faeces diet altered the gut microbiota composition and increased the number of potential pathogens and antibiotic-resistant bacteria in the gut to varying degrees. To reduce the risk of diseases, it is necessary to remove residual antibiotics and antibiotic-resistant bacteria in chicken faeces by fermentation or other techniques, before it can be used as a fish feed for grass carp.