Comparative transcriptomic analysis of goose astrovirus genotype 1 and 2 in goose embryonic fibroblasts.

Abstract

Gout in goslings has become widespread and caused huge economic losses for the goose industry. Emerging evidence suggests that goose astrovirus (GAstV) is a prominent etiological factor of gout in goslings. At present, 2 genotypes of GAstV have been identified named GAstV-1 and GAstV-2. Here, we isolated the GAstV-1 HBLY strain and GAstV-2 XT1 strain from HeBei province of China. The genome and proliferation characteristics of GAstV-1 and GAstV-2 were analyzed and the results showed that the whole genome identity was 53.8% to 55.8%, especially the nucleotide and amino acids identity of ORF2 and Cap protein was only 49.5% to 50.5% and 19.6% to 22.6 %. Interestingly, GAstV-1 and GAstV-2 with such low homology both can cause gout in goslings. To further explore this phenomenon, the whole genomic expression profile of goose embryonic fibroblasts (GEFs) infected with GAstV-1 was investigated in comparison with GAstV-2. The results revealed that 126 differentially expressed genes (DEGs) were identified between GAstV-1-infected and uninfected cells at 48 h postinfection (hpi), and 262 DEGs between GAstV-2 and uninfected. Among these, there are 15 commonly up-regulated genes and 19 commonly down-regulated genes. Gene ontology (GO) enrichment analysis, Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis, and short time-series expression miner (STEM) analysis suggested that GAstV-1 can induce a higher innate immune response to GEFs, while GAstV-2 has a more pronounced effect on GEFs metabolic pathways. The transcriptomic analysis results significantly enhance our comprehension of the pathogenic mechanisms of GAstV.