Comparative whole blood late-stage transcriptomics of polysaccharide and conjugated meningococcal serogroup A vaccine reveals distinct and persistent immune signatures in murine model.

Abstract

Meningococcal Serogroup A conjugate vaccine, as compared to the polysaccharide vaccine, has been clinically proven to induce superior protective immune responses against serogroup A infection in infants and young children. Studies examining the immune pathways/biomarkers responsible for the superiority of conjugate over plain polysaccharide vaccines are greatly required. This study reports the comparative whole blood transcriptomic profiles of the late-stage immune responses induced by meningococcal polysaccharide and conjugate vaccine in a murine model. Unique peripheral gene transcripts were induced by the Men A PS-tetanus toxoid (Men A PS-TT) vaccine compared to the Men A PS-alum (Men A PS-ALPO4) vaccine. Conjugate vaccine reported significant expression for Actb, Cd1d1, Klra8, Klrd1, Tnf, and H2-Q10 genes. Whereas plain polysaccharide vaccine reported key changes for Itga2b, H2-Ab-1, Cd8b1, and Icam2 genes. Annotation studies reported markers related to cytokine and chemokine activation, T and B cell activation, NKT-cell mediated cytotoxicity, antigen processing and presentation, and complement activation were differentially expressed in the conjugate vaccine as compared to the polysaccharide vaccine. The study reports late-stage immune markers that could be potential biomarkers for a better understanding of pathways responsible for immunity to T cell-dependent and independent antigens.