Comparison between antibacterial effect of nanoparticles and non-nanoparticles of chitosan on Listeria monocytogenes isolated from different types of Egyptian cheese

Abstract

The objectives of this paper were to investigate the existence of Listeria monocytogenes (L. monocy-togenes) in different types of cheese (Mozzarella, Thalaga, and Romy cheeses, 40 samples each) sold in Assiut dairy shops and to evalute antibacterial properties of chitosan and its nanoparticles against isolated strains. L. monocytogenes was isolated in higher percentage from Mozzarella cheese (25%) followed by Thalaga and Romy cheese samples. L. monocytogenes isolates were validated by PCR utilising the 16S rRNA gene. Zeta-sizer and TEM were used to characterise nano-chitosan. The mini-mum inhibitory concentrations of chitosan and nano-chitosan on L. monocytogenes were detected by MIC test. Nano-chitosan (3.125%) could prevent organism growth in cheese as early as the third week. The findings revealed that 3.125% nano-chitosan had a sufficient effect on L. monocytogenes while also providing good consumer satisfaction; therefore, nano-chitosan should be utilised as a natural food additive in Egyptian dairy products.