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Peer-reviewed veterinary case report

Comparing UTid+ and standard urine cultures for dog and cat infections

By Stephen D. Cole et al.·Published in Veterinary Sciences·2022·Clinical Microbiology Laboratory, Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA, CH·View original on DOAJ

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Original publication title: Comparison of a Chromogenic Urine Culture Plate System (UTid+) and Conventional Urine Culture for Canine and Feline Specimens

Plain-English summary

A group of dogs and cats with suspected urinary tract infections (UTIs) had their urine tested using a new point-of-care culture system called UTid+ and the traditional urine culture method. Both methods detected infections, but the conventional culture was slightly more effective. The UTid+ system provided results quickly, which can be helpful when a common UTI bacteria is suspected. However, the traditional method is still considered the best option for accurate diagnosis.

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Abstract

In companion animal medicine, urinary tract infection (UTI) is one of the most common indications for antimicrobial therapy. Definitive diagnosis of UTI requires isolation of bacteria with routine urine culture from an animal with concurrent clinical signs. Urine culture is typically performed at reference laboratories where paired susceptibility testing can be performed, but delays in shipment or processing can affect results. This study evaluated the use of a selective chromogenic, point-of-care culture system (UTid+) compared to conventional urine culture. A total of 119 (73 canine and 46 feline) cystocentesis urine samples were evaluated. Conventional urine culture was positive for 28 (23.5%) of the 119 cultures and UTid+ culture was positive for 26 (21.8%). The overall sensitivity, specificity, positive predictive value, negative predictive value and accuracy were 92.3%, 97.8%, 92.3%, 97.8 and 96.6% for UTid+ respectively. Overall, the UTid+ culture system showed an acceptable level of accuracy when compared to conventional urine culture. Agreement of identification results was high (κ = 0.90) with an important exception being <i>Proteus</i> spp. which was only identified in 1/3 positive cultures. UTid+ may be useful in scenarios where a common UTI pathogen is expected and identification within 24 h is ideal; however, conventional urine culture remains the gold standard.

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Original publication on DOAJ: https://doi.org/10.3390/vetsci9030138