Peer-reviewed veterinary case report
Comparison of Gold Nanoparticle Conjugated Secondary Antibody with Non-Gold Secondary Antibody in an ELISA Kit Model.
- Journal:
- Monoclonal antibodies in immunodiagnosis and immunotherapy
- Year:
- 2015
- Authors:
- Emami, Tara et al.
- Affiliation:
- 1 Department of Proteomics and Biochemistry
- Species:
- bird
Abstract
In this study, gold nanoparticles (AuNPs) were used as carriers of the signaling anti-chicken antibody peroxidase in comparison with anti-chicken antibody peroxidase without gold nanoparticle in a commercial avian influenza kit. AuNPs enhanced the absorbance and shortened the assay time. AuNPs act as a carrier of many enzymes and multiply the effect of enzyme when reacting with substrate. They amplify optical signal, while keeping low background signals.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26492626/