Comparison of the effects of direct and remote ischemic post and preconditioning in the kidney of young rats: histological and laboratory evaluation.

Abstract

PURPOSE: To evaluate the effects of direct and remote ischemic preconditioning and postconditioning on kidney ischemia-reperfusion injury in young rats. METHODS: This study was divided into two experimental phases: preconditioning and postconditioning. In each phase, 78 rats were randomly assigned to four groups: control (CG), ischemia-reperfusion (IRG), direct ischemic pre/postconditioning (DIPG), and remote ischemic pre/postconditioning (RIPG). Animals were euthanized at 0- or 24-hours post-procedure, and samples were collected for histological and biochemical analyses. RESULTS: The sum of the histological findings revealed no significant differences among the pre/postconditioning groups. However, serum calcium levels were significantly higher in preconditioning, DIPG compared to the control group (p = 0.002), and in postconditioning, DIPG and RIPG compared to the control group (p = 0.028 and p = 0.005, respectively). In postconditioning, the chlorine levels were higher in IRG compared to the DIPG group (p = 0.029). CONCLUSION: In our findings, ischemic preconditioning and ischemic postconditioning no provided significant protection of renal tissue.