Comparison of the IDEXX ProCyte One to the ProCyte Dx and ADVIA 120 in Dogs and Cats.

Abstract

BACKGROUND: The ProCyte One is a veterinary hematology analyzer. It uses laser flow cytometry with four optical detectors to categorize RBCs, WBCs, and platelets. OBJECTIVE: We aimed to compare results from the ProCyte One with the ProCyte Dx and ADVIA 120, as well as the results of manual PCVs and WBC differential counts, to determine their performance for automated CBCs in dogs and cats. MATERIALS AND METHODS: ProCyte One inter-assay and intra-assay precision were assessed using control material and patient samples. For analyzer comparison, patient samples were run on all three analyzers within 2&#x2009;h of each other. Passing-Bablok regression and visual inspection of Bland-Altman plots were used to determine analyzer congruence, and Spearman's rank correlation was performed. RESULTS: Precision was generally excellent, with CV <&#x2009;4% for most measurands except for reticulocytes, lymphocytes, monocytes, and eosinophils. A total of 140 canine and 96 feline samples were included for the comparison study. Correlations were &#x2265;&#x2009;0.93 for all comparisons except mean cell volume (MCV), mean cell hemoglobin concentration (MCHC), reticulocytes, lymphocytes, monocytes, and eosinophils. Statistically significant bias was suggested by Passing-Bablok regression for many measurands, but most were minor except for MCV compared to ADVIA 120, monocytes compared to all methods, and RBC/hematocrit in anemic dogs. CONCLUSIONS: The ProCyte One demonstrates acceptable agreement with ProCyte Dx and ADVIA 120. However, because of the potential for significant differences between methods, operators should exercise caution when comparing patient results between different analyzers.