Comparison of the therapeutic effect of platelet-rich plasma and injectable platelet-rich fibrin on testicular torsion/detorsion injury in rats.

Abstract

Testicular torsion is a common disorder in males and results in blockage of testicular circulation with subsequent damage of testicular germ cells. The current work aimed to compare the therapeutic effect of platelet-rich plasma (PRP) and injectable platelet-rich fibrin (i-PRF) on torsion/detorsion (T/D) injury in rats. Forty mature male Wister rats were arranged into 4 groups; (1) Control, (2) T/D, (3) T/D&#x2009;+&#x2009;PRP, and (4) T/D+&#x2009;i-PRF. The right testis was twisting 1080&#xb0; clockwise for 3&#xa0;h in groups 2, 3 and 4, then 10&#xa0;&#x3bc;l of PRP or i-PRF was injected intra-testicular 3&#xa0;h after detorsion in groups 3 and 4, respectively. After 30 days postoperatively, the semen quality and hormonal assay were improved in PRP and i-PRF-treated groups with superiority of i-PRF (P&#x2009;<&#x2009;0.001). High significance of Catalase, Glutathione Peroxidase (GPx), Superoxide Dismutase, Interleukin-1&#x3b2; (IL-1&#x3b2;), Caspase-3 and Tumor necrosis factor-&#x3b1; (TNF-&#x3b1;) was reported in treated rats with PRP and i-PRF (P&#x2009;<&#x2009;0.001) with superiority to i-PRF-treated rats (P&#x2009;<&#x2009;0.001). Testicular histoarchitectures were improved in PRP and i-PRF-treated rats with superiority of i-PRF-treated rats. It was concluded that PRP and i-PRF have regenerative efficacy on testicular damage after induced T/D injury with a superior efficacy of i-PRF.