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Peer-reviewed veterinary case report

Comparison of two PCR systems for the rapid detection of Leptospira spp. from kidney tissue.

Journal:
Current microbiology
Year:
2011
Authors:
Mayer-Scholl, Anne et al.
Affiliation:
Federal Institute for Risk Assessment (BfR) · Germany

Abstract

In this study we compared two routine PCR systems for the detection of Leptospira spp. and assessed their performance when directly applied to kidney samples from small mammals. Although the kappa value of 0.9 indicated a high level of agreement between the tests, the outer membrane lipoprotein gene lipl32 based PCR was more robust and showed a higher number of positive kidney samples.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/21132297/