Comparison ofDNA Isolation from Swabs Without Pre-Enrichment on Selective Media With Isolation Preceded by Warm and Cold Enrichment.

Abstract

Bacteriological methods for the identification ofare laborious and time-consuming. The aim of this study was to compareDNA isolation from swabs without pre-enrichment on selective media with isolation preceded by warm and cold enrichment. The material for the study consisted of 150 rectal swabs taken from 50 clinically healthy fattening pigs. Forty-onestrains were isolated after warm enrichment and 43 after cold enrichment. The presence of,, andgene fragments was detected in all isolates. DNA isolation from swabs without pre-enrichment supported the detection ofin only nine samples. This study demonstrates thatDNA can be isolated from swabs without pre-enrichment on selective media, but this method is less sensitive than the approach where DNA isolation is preceded by warm or cold enrichment.