Peer-reviewed veterinary case report
Amniotic membrane storage times and healing of dog corneal ulcers
By Dower, Nathalie Moro Bassil et al.·Published in Veterinary ophthalmology·2022·Dower Oftalmovet, Brazil·View original on PubMed →
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Original publication title: Concentrations of tissue inhibitor of matrix metalloproteinase-1 and hyaluronic acid in canine amniotic membranes cryopreserved for different time points and its effects in dogs with complicated corneal ulcers.
- Species:
- dog
Plain-English summary
A group of dogs with serious corneal ulcers underwent surgery to repair their eyes using amniotic membranes (AMs) that had been frozen for different lengths of time. The results showed that AMs stored for a shorter time led to faster healing and less cloudiness in the eyes compared to those stored longer. While the levels of certain proteins in the AMs changed over time, the overall success of the surgeries remained good even with AMs that had been frozen for up to a year. Most dogs regained some vision after treatment, especially those with deep ulcers.
People also search for: dog corneal ulcer treatment · amniotic membrane for dog eye problems · dog eye surgery recovery time
Abstract
OBJECTIVES: To determine the concentrations of total protein (TP), tissue inhibitor of matrix metalloproteinase-1 (TIMP-1) and hyaluronic acid (HA) in amniotic membranes (AMs) harvested from placentas of bitches of different ages and cryopreserved for different time points. The outcomes of complicated corneal defects of dogs repaired with AMs stored for the same time points were also evaluated. PROCEDURES: Ten cryopreserved canine AMs were stored for short term (2-50 days), middle term (92-210 days), or long term (256-357 days). TP was quantified by Bradford's test, whereas TIMP-1 and HA were quantified by ELISA. Twenty-one dogs that had an AM transplantation to restore deep or perforating corneal wounds were selected. RESULTS: TIMP-1 levels were lower in AMs cryopreserved for middle term (p = .02) and long term (p = .0009), when compared to AMs stored for short term. TP (p = .39) and HA (p = .18) concentrations in AMs did not differ among the storage time. TIMP-1 concentration in AMs correlated with storage time (R = -.65, p < .0001), while TP (R = -.33, p = .07) and HA concentrations did not (R = -.15, p = .41). The age of donors did not correlate with the components evaluated in the AMs. Corneal defects repaired with AMs stored for short term healed sooner than the ones repaired with AMs stored for middle (p < .01) and long term (p = .02). Additionally, TIMP-1 levels in AMs correlated negatively with the epithelization time (R = -.62, p = .002). Graft opacity was severe in 55% of cases. However, the HA levels in AMs correlated negatively with the opacification score (R = -.47, p = .03). Vision was observed in more patients who presented deep ulcers and descemetoceles, than in the ones with perforations (p = .004). CONCLUSIONS: TIMP-1 concentration in canine AMs significantly decreased over a year storage time, while TP and HA concentrations did not change during the same period. The age of donors did not correlate with the components evaluated in the AMs. Complicated corneal defects repaired with AMs cryopreserved for short term healed sooner and tended to be less opaque; however, satisfactory to optimal outcomes were achieved even in the eyes repaired with AMs stored for up to a year.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/34240563/