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Peer-reviewed veterinary case report

Confirmation of sex-specific transcripts from Ancylostoma caninum adult worms by semi-quantitative RT-PCR.

Journal:
Research in veterinary science
Year:
2007
Authors:
Miranda, R R et al.
Affiliation:
Departamento de Parasitologia · Brazil

Abstract

In a previous work Ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific RT-PCR comparing male and female cDNA. In this work more fragments had been identified and a semi-quantitative RT-PCR carried out in order to confirm the sex-specific character of the transcripts obtained. Four fragments were confirmed as sex-specific or as being expressed more in one sex than the other.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/17005221/