Constructing of Synthetic DNA-Histone Networks as Extracellular Trap Model for Biomedical Research.

Abstract

Extracellular traps (ETs) released during ETosis of various blood cells not only participate in host defense against pathogenic microorganisms, but also play an essential role in diverse pathological processes. It should be noted that ETs are heterogeneous by their structure; therefore, for studying their pathophysiological effects, it is extremely important to create model ETs with strictly controlled composition and structure. We propose an approach that allows creating model mesh structures based on DNA and histones (DNA-histone networks, DHN), which can be considered as model ETs. It has been shown that the structure of DHN depends on the DNA/histone weight ratio. The possibility of incorporating myeloperoxidase that can be present in native ETs, into DHN has been demonstrated. The creation of DHN with controlled composition and structure will presumably allow assessing the specificity of enzyme systems involved in the degradation of native ETs. In addition, these heterogeneous DHN can be used to study the relationship between ET structure and ET-induced thrombosis or their antimicrobial activity.