Peer-reviewed veterinary case report
Construction and Application of Newcastle Disease Virus-Based Vector Vaccines.
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2016
- Authors:
- Wichgers Schreur, Paul J
- Affiliation:
- Department of Virology · Netherlands
Abstract
Paramyxoviruses are able to stably express a wide-variety of heterologous antigens at relatively high levels in various species and are consequently considered as potent gene delivery vehicles. A single vaccination is frequently sufficient for the induction of robust humoral and cellular immune responses. Here we provide detailed methods for the construction and application of Newcastle disease virus (NDV)-based vector vaccines. The in silico design and in vitro rescue as well as the in vivo evaluation of NDV based vectors are described in this chapter.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/26458839/