Peer-reviewed veterinary case report
Detecting DNA changes in dog bladder cancer with digital PCR
By Sakai, Kosei et al.·Published in Veterinary pathology·2020·Department of Veterinary Clinical Pathobiology, Japan·View original on PubMed →
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Original publication title: Copy Number Aberration in Canine Urothelial Carcinoma Detected by a Digital Polymerase Chain Reaction Assay.
- Species:
- dog
Plain-English summary
A study found that a digital test could help detect a common bladder tumor called urothelial carcinoma (UC) in dogs. Researchers looked at tissue and urine samples from dogs with UC and found that about one-third of the bladder tissues and over a third of the urine samples showed changes in DNA that could indicate the presence of the tumor. This test could be a helpful, non-invasive way for veterinarians to identify UC in dogs, which is important for early diagnosis and treatment.
People also search for: dog bladder cancer symptoms · urothelial carcinoma in dogs · non-invasive bladder cancer test for dogs
Abstract
Urothelial carcinoma (UC) is the most common tumor affecting the urinary bladder of dogs. Protein overexpression of ErbB2 (the canine homolog of HER2) has been observed in dogs with UC. However, no study regardingcopy number aberration (CNA) is reported in dogs with UC. In this study, a digital PCR assay for detecting CNA of caninewas developed. DNA samples were isolated from 83 formalin-fixed, paraffin-embedded urinary bladder tissues (36 UC, 8 polypoid cystitis, and 39 normal) and 94 urinary sediments (54 UC, 30 nonneoplastic, and 10 normal). The copy number of canine chromosome 8 () was used as a control. In the urinary bladder tissues,CNA was detected in 12 of 36 (33%) UC, 2 of 8 (25%) polypoid cystitis, and 0 of 39 (0%) normal controls. In the urinary sediments,CNA was also detected in 19 of 54 (35%) UC; however, noCNA was detected in nonneoplastic diseases or normal controls. The sensitivity and specificity ofCNA in urinary sediment for the detection of UC were 35% and 100%, respectively. There was a positive correlation between the copy number ratios oftoin the urinary bladder tissues and urinary sediments. Our findings indicate that the digital PCR assay of urinary sediments may be a useful, noninvasive method for detectingCNA in dogs with UC.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/31640537/