Peer-reviewed veterinary case report
Cost-effective method of DNA extraction from taeniid eggs.
- Journal:
- Parasitology research
- Year:
- 2008
- Authors:
- Dyachenko, V et al.
- Affiliation:
- Institute of Parasitology · Germany
- Species:
- dog
Abstract
A new cost-effective method using silicon dioxide- and guanidine isothiocyanate-containing buffers, after previous alkaline lysis, was established for the DNA extraction from taeniid eggs isolated from canine faeces. The purified DNA can be used to amplify the species-specific 12S mitochondrial DNA of Echinococcus multilocularis in direct and nested polymerase chain reaction in order to differentiate between E. multilocularis and Taenia spp.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/18172686/