Peer-reviewed veterinary case report
Creation of primary cell lines from lineage-labeled mouse models of cancer.
- Journal:
- Cold Spring Harbor protocols
- Year:
- 2015
- Authors:
- Rhim, Andrew D
- Affiliation:
- Department of Internal Medicine · United States
Abstract
Frequently, it is necessary to isolate pure populations of cancer cells for downstream assays, such as transcriptional analysis, signaling studies, and the creation of noncontaminated primary cell lines. Genetic lineage labeling with fluorescent reporter alleles allows for the identification of epithelial-derived cells within tumors. This protocol describes a method to isolate lineage-labeled pancreatic epithelial cells for ex vivo analysis, but it can be adapted for any type of lineage-labeled tumor.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25934932/