CRISPR/cas9 cassette targetingCTX-M specific gene of mastitis cow milk origin can alter the antibiotic resistant phenotype for cefotaxime.

Abstract

CTX-M beta-lactamases are one of the most important extended spectrum beta-lactamase (ESBL) resistance enzymes found in. In the present study, 59% ofisolates from mastitis cow milk were reported to be positive for ESBL types. The prevalence of beta-lactam (β-lactam) antibiotic resistance was reported to be 84%, 72.7%, 52.27%, 50%, and 45.4% for cefotaxime, cefepime, cefuroxime, oxacillin, and cephalexine, respectively. TheCTX-M gene was found in 65% ( = 17) of theisolates when they were genotyped. Further, the use of a CRISPR/cas9 cassette to target theCTX-M gene revealed changes in antibiotic phenotypes for cefotaxime.