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Peer-reviewed veterinary case report

Critical diagnostic and cancer stem cell markers in neoplastic cells from canine primary and xenografted pulmonary adenocarcinoma.

Journal:
Journal of veterinary science
Year:
2022
Authors:
Tangchang, Warisraporn et al.
Affiliation:
College of Veterinary Medicine and Institute of Veterinary Science · South Korea

Abstract

It is challenging to diagnose metastatic tumors whose cellular morphology is different from the primary. We characterized canine primary pulmonary adenocarcinoma (PAC) and its xenografted tumors by histological and immunohistochemical analyses for critical diagnostic and cancer stem cell (CSC) markers. To generate a tumor xenograft model, we subsequently transplanted the tissue pieces from the PAC into athymic nude mice. Immunohistochemical examination was performed for diagnostic (TTF-1, Napsin A, and SP-A) and CSC markers (CD44 and CD133). The use of CSC markers together with diagnostic markers can improve the detection and diagnosis of canine primary and metastatic adenocarcinomas.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/36448435/