Peer-reviewed veterinary case report
Cross-reactivity of chicken anti-Japanese encephalitis virus serum and anti-West Nile virus serum in serological diagnosis.
- Journal:
- The Journal of veterinary medical science
- Year:
- 2012
- Authors:
- Hirota, Jiro et al.
- Affiliation:
- National Institute of Animal Health · Japan
Abstract
The cross-reactivity of Japanese encephalitis virus (JEV)-immunized chicken sera and West Nile virus (WNV)-immunized chicken sera in serological tests, such as the IgG indirect ELISA (IgG-ELISA), hemagglutination inhibition test (HI) and plaque reduction neutralization test (PRNT), for JEV and WNV were examined. The mean JEV/WNV ELISA ratio in IgG-ELISA of JEV-immunized sera was significantly higher than that of WNV-immunized sera (P<0.01). JEV-immunized chicken sera did not cross-react in the WNV HI. However, all the WNV-immunized chicken sera cross-reacted in the JEV HI. JEV-immunized chicken sera did not show the WNV neutralization titer at 90% plaque reduction, and WNV-immunized chicken sera did not showed the JEV neutralization titer at 90% plaque reduction. Therefore, it is possible that chicken JEV serum can be distinguished from WNV serum by comparing the titers of IgG-ELISA, HI or PRNT respectively.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/22785078/