Peer-reviewed veterinary case report
CyTOF protocol for immune monitoring of solid tumors from mouse models.
- Journal:
- STAR protocols
- Year:
- 2023
- Authors:
- Shin, Sarah M et al.
- Affiliation:
- Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins · United States
Abstract
Techniques for robust immune profiling of mouse tumor and blood are key to understanding immunological responses in mouse models of cancer. Here, we describe mass cytometry (cytometry by time-of-flight) procedures to facilitate high-parameter profiling of low-volume survival blood samples and end-of-study tumor samples. We employ live-cell barcoding systems to mark all cells from each tumor and blood to improve cost-effectiveness and minimize batch effects. For complete details on the use and execution of this protocol, please refer to Charmsaz et al. (2021)..
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/36538397/