Deciphering pathogenicity and virulence of the firstisolate from diabetic foot osteomyelitis.

Abstract

INTRODUCTION: This study identifiesas a new coagulase-negative staphylococcal species isolated from diabetic foot osteomyelitis (DFOM) and provides an in-depth analysis of its pathogenic and virulence profile, as well as demonstrating its potential to cause infection. METHODS: TheNSD001 strain was examined for its planktonic growth, biofilm production, and phagocytosis rates in murine macrophages compared toNSA739. Additionally, persistence and replication within human osteoblasts were investigated, while the zebrafish embryo model was employed to assess virulence. Genomic sequencing and bioinformatic analysis were also conducted to identify genes associated with virulent potential. RESULTS AND DISCUSSION: NSD001 exhibited robust planktonic growth and significant biofilm production, highlighting its capacity to initiate and maintain an infection, and demonstrated similar rates of phagocytosis asNSA739 in murine macrophages, suggesting a mechanism for evading initial host defenses. The strain persisted and replicated within human osteoblasts, indicative of a strategy for intracellular survival and facilitation of chronic osteomyelitis. The zebrafish embryo model revealed a slower, yet fatal, virulence profile forNSD001 compared to the rapid lethality induced byNSA739. Genomic sequencing and bioinformatic analysis uncovered various genes corroborating its virulence.NSD001 poses a significant concern in DFOM due to its ability to form biofilms and survive within host cells, presenting challenges for current treatment strategies. This underscores the need for updated clinical protocols and increased awareness among healthcare professionals to effectively manage infections caused by this emerging pathogen.