Decreased placental X-linked inhibitor of apoptosis protein in an ovine model of intrauterine growth restriction.

Abstract

OBJECTIVE: The objective of the study was to assess placental apoptosis at both midgestation and near term in an ovine model of placental insufficiency (PI) and intrauterine growth restriction (IUGR). STUDY DESIGN: At 40 days' gestational age (dGA), 2 groups of 4 ewes were exposed to hyperthermic conditions for either 55 days or 80 days to induce IUGR with necropsies at 95 (midgestation) and 130 dGA (term = 140 dGA), respectively. Blood gases were assessed and placental tissues obtained for apoptosis analyses. RESULTS: PI-IUGR pregnancies showed: (1) a decrease in fetal O(2) saturation and pO(2) (P < .04), (2) an increase in placental villi apoptosis (P < .05) at midgestation and near term, and (3) a decrease of cotyledon X-linked inhibitor of apoptosis protein (XIAP) at both gestational periods (P < .04) with no differences in caruncle XIAP protein. CONCLUSION: Placental villous apoptosis is increased at midgestation and near term in our ovine model of IUGR, and this increase is associated with a significant decrease in XIAP protein in the cotyledon of IUGR animals.