Deletion of the Transcriptional Regulator MucR inAffects Stress Responses and Bacterial Virulence.

Abstract

The transcriptional regulator MucR is related to normal growth, stress responses andvirulence, and affects the expression of various virulence-related genes in smooth-typestrains. However, the function of MucR in the rough-typeremains unknown. In this study, we discovered that MucR protein was involved in resistance to heat stress, iron-limitation, and various antibiotics in.. In addition, the expression level of various bacterial flagellum-related genes was altered inmutant strain. Deletion of this transcriptional regulator insignificantly affectedvirulence in RAW264.7 macrophage and mice infection model. To gain insight into the genetic basis for distinctive phenotypic properties exhibited bymutant strain, RNA-seq was performed and the result showed that various genes involved in translation, ribosomal structure and biogenesis, signal transduction mechanisms, energy production, and conversion were significantly differently expressed in Δstrain. Overall, these studies have not only discovered the phenotype ofmutant strain but also preliminarily uncovered the molecular mechanism between the transcriptional regulator MucR, stress response and bacterial virulence in..