Peer-reviewed veterinary case report
Deletion of YTHDF1 (not YTHDF3) reduced brain and gut damage after traumatic brain injury.
- Journal:
- Neurological research
- Year:
- 2024
- Authors:
- Zhao, Wei et al.
- Affiliation:
- Department of Neurosurgery · China
- Species:
- rodent
Abstract
OBJECTIVE: To determine whether YTHDF1 and YTHDF3 play the same role in brain and gut damage after traumatic brain injury (TBI). METHODS: We generated YTHDF1-/- and YTHDF3-/- mice using CRISPR/Cas9 technology, established a mouse brain injury model through severe controlled cortical impact (CCI), and finally observed the different types of damage between YTHDF1-/- and YTHDF3-/- mice by analysing the levels of oedema proteins in cortical tissue and inflammatory proteins and histopathological lesions in brain and gut tissues in mice at 3 days after CCI. RESULT: Compared with WT mice, YTHDF1-/- mice had decreased levels of oedema in cortical tissue and inflammation and histopathological lesions in brain and gut tissues at 3 days post-CCI, but YTHDF3-/- mice did not. CONCLUSION: Our results suggest that deletion of YTHDF1, but not YTHDF3, could reduce damage to the brain and gut following TBI.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/39081030/