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Peer-reviewed veterinary case report

Delivery of antisense peptide nucleic acid by gold nanoparticles for the inhibition of virus replication.

Journal:
Nanomedicine (London, England)
Year:
2019
Authors:
Ghaffari, Elnaz et al.
Affiliation:
Department of Biology

Abstract

We aim to use peptide nucleic acid (PNA) for antisense therapy against bovine viral diarrhea virus (BVDV), a surrogate model of human hepatitis C virus, and introduce an optimal approach for delivering PNA into the cell.PNA was designed for hybridization to the 5'-untranslated region of BVDV RNA in order to form a heteroduplex structure and inhibit the translation and replication of virus. Gold nanoparticles (AuNPs) were used as a delivery system for PNA.The cellular uptake of PNA-AuNPs and inhibition of BVDV infection in the middle stage of viral replication were found.Further research is warranted to develop AuNPs as a potential vehicle for delivering PNA in order to remove viruses from the infected cells.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/31274375/