Peer-reviewed veterinary case report
IgG antibody changes in atopic dogs treated with dust mite allergy
By Hou, Chia-Chun et al.·Published in Veterinary dermatology·2008·The Royal Dick School of Veterinary Studies, United Kingdom·View original on PubMed →
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Original publication title: Dermatophagoides farinae-specific IgG responses in atopic dogs undergoing allergen-specific immunotherapy with aqueous vaccines.
- Species:
- dog
Plain-English summary
A group of 15 dogs with skin allergies caused by dust mites were treated with allergen-specific immunotherapy (ASIT) using special vaccines for at least two months. Before starting treatment, all dogs had high levels of specific antibodies against the dust mite, Dermatophagoides farinae. During the therapy, the dogs showed a significant increase in these antibodies, which suggests that the treatment was effective in helping their immune systems respond better to the allergens. This means that ASIT may help reduce the allergic reactions in dogs suffering from atopic dermatitis caused by dust mites.
People also search for: dog skin allergies treatment · atopic dermatitis in dogs · dust mite allergy in dogs
Abstract
The molecular and immunologic mechanisms associated with successful allergen-specific immunotherapy (ASIT) have not been completely elucidated. The aim of this study was to characterize the changes in Dermatophagoides farinae-specific IgG in atopic dogs undergoing ASIT using aqueous vaccines. Fifteen atopic dogs with a positive skin test reaction to D. farinae were treated with aqueous vaccines for a minimum of 2 months following a standard protocol. Serum samples were collected before and during therapy and used to probe Western blots containing separated proteins of D. farinae. IgG responses were detected using a polyclonal goat anticanine IgG antibody and a chromogenic substrate 3,3'-diaminobenzidine. The blots were analysed using a semiquantitative digital image analysis system that evaluated the number and molecular weight of bands, as well as their intensity, which was related to IgG concentration. Prior to ASIT, all dogs showed allergen-specific IgG responses to various antigens of D. farinae. During ASIT, there was a significant increase in the total quantity of D. farinae-specific IgG antibodies to various antigens from the mite (P = 0.015). Significant increases were observed for a 98-kDa band (P = 0.015), likely to be Der f 15; bands with molecular weights between 50 and 70kDa (P=0.012); and bands between 30 and 45 kDa (P = 0.035). These findings provide support for the hypothesis that ASIT induces IgG blocking antibodies to allergens known to be relevant in canine atopic dermatitis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/19086120/