Peer-reviewed veterinary case report
Detecting Candida yeast in urine of dogs with cystitis
By Kano, Rui et al.Ā·Published in The Journal of veterinary medical scienceĀ·2002Ā·Department of Pathobiology, JapanĀ·View original on PubMed ā
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Original publication title: Detection and identification of the Candida species by 25S ribosomal DNA analysis in the urine of candidal cystitis.
- Species:
- dog
Plain-English summary
Two dogs with chronic bladder infections were brought to a veterinary clinic for evaluation. Tests on their urine samples showed the presence of yeast cells, which were identified as Candida albicans and C. parapsilosis, common causes of urinary tract infections. A new testing method using PCR analysis on their urine allowed for quick identification of these yeast species within two days. This approach could help veterinarians diagnose and treat similar infections more efficiently in the future.
People also search for: dog urinary tract infection symptoms Ā· Candida albicans in dogs Ā· dog bladder infection treatment
Abstract
Candida species in clinical urine samples were identified directly by the newly developed method of PCR analysis on 25S ribosomal DNA (rDNA). Two dogs were referred to the Animal Medical Center, Nihon University School of Veterinary Medicine, Fujisawa, Kanagawa, Japan for the examination of chronic cystitis. Microscopic examination of urine samples from these dogs revealed yeast cells. Urine culture on Sabouraud's dextrose agar at 27 degrees C for 5 days produced white to cream colored colonies. The isolates were identifical to Candida albicans and C. parapsilosis by mycological examination, respectively. The nucleotide sequences of 25S ribosomal DNA from these urine isolates showed 99% similarity to those of a reference strain of Candida albicans or C. parapsilosis. The nucleotide sequences of 25S rDNA obtained directly from urine samples were also identical to C. albicans and C. parapsilosis, respectively. Confirming the results on the isolates cultured from the same urine samples. This PCR analysis method could be available for the direct identification of Candida species in urine samples within 2 days.
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Search related cases āOriginal publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/11913546/