Peer-reviewed veterinary case report
Astrovirus and bocavirus found in dogs with gastroenteritis
By Sobhy, N M et al.·Published in Comparative immunology, microbiology and infectious diseases·2022·Veterinary Diagnostic Laboratory and Department of Veterinary Population Medicine, United States·View original on PubMed →
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Original publication title: Detection and molecular characterization of astro and bocaviruses in dogs in Minnesota.
- Species:
- dog
Plain-English summary
Two dogs in Minnesota died from severe gastroenteritis, which is inflammation of the stomach and intestines. Tests on their fecal samples revealed they were infected with canine astrovirus (CAstV) and canine bocavirus (CBoV), both of which can cause gastrointestinal issues in dogs. Out of ten dogs tested, six had CAstV and eight had CBoV, with four dogs infected by both viruses. The study emphasizes the value of advanced testing methods for identifying these viruses in sick dogs.
People also search for: dog diarrhea causes · gastroenteritis in dogs treatment · canine astrovirus symptoms · bocavirus in dogs · dog stomach virus treatment
Abstract
Canine astrovirus (CAstV) and canine bocavirus (CBoV) are involved in cases of mild, and sometimes severe, gastroenteritis in dogs. Fecal samples from two dead dogs with gastroenteritis were received at the University of Minnesota Veterinary Diagnostic Laboratory to determine the cause of death. Small round viruses of 20-35 nm diameter were observed by negative contrast electron microscopy. The samples were subjected to Illumina MiSeq sequencing. Both samples were strongly positive for CAstV; all viral reads were related to CAstV. In addition, sample number 1 had a few reads of CBoV. Two complete sequences of CAstV were identified (6625 and 6627 nt in length) with 95% nt identity. RT-PCR and PCR were used to confirm CAstV and CBoV infections in successive samples of canine gastroenteritis. Sanger sequencing was done on nucleic acids from positive samples. Of a total of ten samples, CAstV and CBoV infections were confirmed in six and eight animals, respectively. Four animals had mixed infection with both viruses. All sequences of ORF1b gene of CAstVs showed closest clusters in phylogenetic tree with 96-100% nucleotide and amino acids identity. On the other hand, identity between VP2 gene of different CBoV strains in this study ranged from 93%- 100%. All strains were located close to each other except the divergent MT078234 strain, which was arranged in a separate branch and was closer to reference strain JN648103/USA/2010. This study highlights the importance of electron microscopy and next generation sequencing for early detection and characterization of viruses associated with dog gastroenteritis.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/34971935/