Peer-reviewed veterinary case report
Detection of activated caspase-8 in injured spinal axons by using fluorochrome-labeled inhibitors of caspases (FLICA).
- Journal:
- Methods in molecular biology (Clifton, N.J.)
- Year:
- 2015
- Authors:
- Barreiro-Iglesias, Antón & Shifman, Michael I
- Affiliation:
- School of Biomedical Sciences · United Kingdom
Abstract
Here, we present a detailed protocol for the detection of activated caspase-8 in axotomized axons of the whole-mounted lamprey spinal cord. This method is based on the use of fluorochrome -labeled inhibitors of caspases (FLICA) in ex vivo tissue. We offer a very convenient vertebrate model to study the retrograde degeneration of descending pathways after spinal cord injury.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25431075/