Peer-reviewed veterinary case report
Detection of Antibodies Against Toxoplasma gondii in Indian Cattle by Recombinant SAG2 Enzyme-Linked Immunosorbent Assay.
- Journal:
- Acta parasitologica
- Year:
- 2019
- Authors:
- Sudan, Vikrant et al.
- Affiliation:
- Indian Veterinary Research Institute (IVRI) · India
Plain-English summary
This study looked at the presence of antibodies against Toxoplasma gondii, a parasite that can affect cattle, in adult cows from Kerala, India. Researchers tested blood samples from 258 cows using a special lab test that detects these antibodies. They found that 61.5% of the cows had antibodies indicating they had been exposed to the parasite. The new test they developed was quite accurate, showing good agreement with another established test. Overall, the findings suggest that Toxoplasma gondii is common in these cattle, which could be important for public health.
Abstract
BACKGROUND: A large number of recombinant proteins are tested over a period of time for diagnosis of toxoplasmosis throughout the world. However, such literature is very much limited from an Indian perspective. PURPOSE: The aim of the study was to assess the sero prevalence of Toxoplasma gondii in adult cattle from Kerala, India, using recombinant surface antigen 2 (recSAG2) protein. METHODS: An antibody detection recombinant ELISA specific for T gondii was laboratory-standardized using recSAG2 protein. The optimum antigen concentration, serum concentration, and conjugate dilutions were determined by the initial checkerboard titrations. Subsequently, the diagnostic potential of the recombinant protein was assessed with 258 field sera samples cattle and compared with indirect fluorescent antibody test (IFAT). RESULTS: Among the cattle sera tested, 61.5% showed sero positivity of T. gondii-speciic IgG. When compared to IFAT, the sensitivity of the recSAG2 ELISA was found to be 80.00% with 88.57% specificity at 95% confidence interval with substantial agreement between the tests. CONCLUSION: The results of present study have revealed the presence of high seroprevalence of the parasite and, hence, immense public health significance.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/30637561/