Peer-reviewed veterinary case report
Detection of antibodies to the biofilm exopolysaccharide of Histophilus somni following infection in cattle by enzyme-linked immunosorbent assay.
- Journal:
- Clinical and vaccine immunology : CVI
- Year:
- 2014
- Authors:
- Pan, Yu et al.
- Affiliation:
- Virginia-Maryland Regional College of Veterinary Medicine · United States
Plain-English summary
Researchers created a test to find antibodies in cattle that indicate an infection with Histophilus somni, a bacteria that can cause illness. This test, called an enzyme-linked immunosorbent assay (ELISA), showed that it could accurately identify infected calves about three weeks after they got sick, with even more positive results by the fourth week. It was also good at correctly identifying healthy calves, meaning it had a low chance of giving false positives. This test could be helpful for veterinarians in diagnosing diseases caused by H. somni in calves.
Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed to detect bovine antibodies to Histophilus somni exopolysaccharide (EPS), which is created during biofilm formation. When an index value of 0.268 was used, the sensitivity of the assay for infected calves was 90.5% at 3 weeks postinfection, but the number of positive animals increased by week 4. The specificity of the assay for healthy calves was 92.5%. The EPS ELISA may aid in identifying calves with H. somni diseases.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/25143338/