Detection of Antimicrobial Resistance and Biofilm Production Amongfrom Canine Skin Lesions.

Abstract

AIMS: is an opportunistic pathogen also indicated as one of the major causes of skin infections in dogs. This study aimed to identifyisolated from canine skin lesions, evaluate their antibiotic resistance profile and biofilm production ability. METHODOLOGY: Lesions from 50 rural dogs with different skin lesions were sampled after pyoderma diagnosis by private practices. Bacterial species determination was investigated and susceptibility to nine antimicrobials were determined by means of Kirby-Bauer assay. Then seven antibiotic resistance genes, including, andwere screened by PCR. Moreover, biofilm formation ability of the strains was determined using the microtiter plate assay along with the presence ofgenes. RESULTS: A total of 37 (74%) isolates were identified asAllisolates were resistant to multiple drugs. Resistance to penicillin, amoxicillin-clavulanic acid, and cefazolin were higher than other antimicrobials. All the beta-lactam-resistant isolates carried, whereaswas found in 6 (16.21%) of them. Among tetracycline-resistant strains, the frequency ofanddeterminants were 19 (90.47%) and 21 (100%), respectively. Finally, most cefazolin-resistant strains 31 (91.89%) were positive forgene. The rate of biofilm production ability amongisolates was 35 (94.59%). Furthermore, the presence ofgenes was detected in 35 (100%) and 29 (82.85%) ofstrains, respectively. CONCLUSION: The high rates of antimicrobial resistance and biofilm production ability amongisolates, emphasize the urgent need to use more effective infection control policies and guidelines for antimicrobial use.