Peer-reviewed veterinary case report
Detection of Burkholderia cepacia DNA from artificially infected EDTA-blood and lung tissue comparing different DNA isolation methods.
- Journal:
- Journal of veterinary medicine. B, Infectious diseases and veterinary public health
- Year:
- 2006
- Authors:
- Merk, S et al.
- Affiliation:
- Institut fuer Medizinische Informatik und Biomathematik · Germany
- Species:
- horse
Abstract
Bacterial DNA (Burkholderia cepacia) was prepared from artificially infected equine ethylenediaminetetraacetic acid (EDTA)-blood and lung tissue by using four standard methods (lysis buffer containing proteinase K, phenol/chloroform/isoamylalcohol-extraction, microwave-treatment, heat treatment) and six commercially available kits (Puregene, High Pure PCR Template Preparation Kit, InstaGene, QiaAmp Tissue Kit, DNAzol and Elu-Quik). After a subsequent polymerase chain reaction (PCR), their efficacy and sensitivity were compared. Concerning the detection limits, the simple lysis with a proteinase K-containing buffer led to the best results for EDTA-blood as well as for artificially infected lung tissue.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/16907960/