Peer-reviewed veterinary case report
Detection of CD8T cell-mediated immune responses to bacterial infection in mice.
- Journal:
- STAR protocols
- Year:
- 2021
- Authors:
- Wu, Jun et al.
- Affiliation:
- School of Life Sciences · China
- Species:
- rodent
Abstract
Efficient activation of CD8T cells is critical for bacterial resistance and eradicating malignancy in the body. Here, we present a step-by-step protocol to useexpressing OVA (LmOVA) to stimulate endogenous CD8T cells. We describe the steps for adoptive transfer of OT-I CD8T cells to CD45.1 mice and then detail the steps for detection of the antigen-specific CD8T cells in response to LmOVA. For complete details on the use and execution of this protocol, please refer to Wu et al. (2021).
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/34977671/