Peer-reviewed veterinary case report
Detecting autoantibodies in dogs with pemphigus foliaceus using
By Honda, R et al.Ā·Published in Research in veterinary scienceĀ·2004Ā·Department of Veterinary Internal Medicine, JapanĀ·View original on PubMed ā
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research ā every detail traces back to the citation above. How we work ā
Original publication title: Detection of circulating autoantibodies using living keratinocyte staining on MCA-B1 method in dogs with pemphigus foliaceus.
- Species:
- dog
Plain-English summary
A group of dogs with a skin condition called pemphigus foliaceus (PF), which causes sores and blisters, was tested for specific autoantibodies using a new method involving living skin cells. Out of 27 dogs with PF, 4 tested positive with this method, while other traditional tests showed higher positive rates. The new method was less sensitive but could still help confirm a diagnosis of PF. This means that while it may not catch every case, it can still provide useful information for veterinarians when diagnosing this skin disease.
People also search for: dog skin sores pemphigus foliaceus Ā· dog autoimmune skin disease treatment Ā· how to diagnose pemphigus in dogs
Abstract
In this study, we compared the sensitivity and specificity of three immunofluorescence techniques used to detect circulating autoantibodies in dogs with pemphigus foliaceus (PF); living keratinocyte staining on a canine keratinocyte cell line, MCA-B1, indirect immunofluorescence (IIF) on canine lip and IIF on bovine esophagus. Sera from canine PF cases were positive in four out of 27 dogs (14.8%) using living keratinocyte staining on MCA-B1 cells method, and five (18.5%) and eight sera (29.6%) using IIF on canine lip and bovine esophagus methods, respectively. By contrast, none of the 31 sera from dogs with non-pemphigus dermatoses reacted with MCA-B1 cells, whereas two (6.5%) as well as five sera (16.1%) obtained from those dogs showed positive reactivity with IIF on canine lip and bovine esophagus, respectively. Our results suggest that, although it exhibits the least sensitivity, the positive reactivity obtained by living keratinocyte staining on MCA-B1 cells can support the diagnosis of canine PF.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases āOriginal publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/15196900/