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Peer-reviewed veterinary case report

Detection of Escherichia coli O104 in the feces of feedlot cattle by a multiplex PCR assay designed to target major genetic traits of the virulent hybrid strain responsible for the 2011 German outbreak.

Journal:
Applied and environmental microbiology
Year:
2013
Authors:
Paddock, Z D et al.
Affiliation:
Department of Diagnostic Medicine and Pathobiology · United States

Abstract

A multiplex PCR was designed to detect Escherichia coli O104:H4, a hybrid pathotype of Shiga toxigenic and enteroaggregative E. coli, in cattle feces. A total of 248 fecal samples were tested, and 20.6% were positive for serogroup O104. The O104 isolates did not carry genes characteristic of the virulent hybrid strain.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/23542615/