Peer-reviewed veterinary case report
Detection of granzyme B in CD3-positive cells infiltrated in lesional skin of a dog with erythema multiforme associated with zonisamide.
- Journal:
- The Journal of veterinary medical science
- Year:
- 2021
- Authors:
- Tanaka, Nana et al.
- Affiliation:
- Joint Department of Veterinary Medicine · Japan
- Species:
- dog
Abstract
In human erythema multiforme (EM), cytotoxic T lymphocytes (CTLs) play an essential role in the pathogenesis. In canine EM, immunohistochemical staining with anti-CD8 antibody using frozen sections has shown the involvement of CTLs; however, CTL infiltration has never been quantitatively analyzed. We herein quantitatively analyzed CTL infiltration by immunohistochemical staining with granzyme B and CD3 antibodies using paraffin sections of a dog with EM associated with zonisamide. The present results indicated approximately 70% of cells at the border between the epidermis and dermis consisted of CTLs. Detection of granzyme B and CD3 using paraffin sections employed in this study can be a clinically applicable method for detecting CTLs.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/34433730/