Peer-reviewed veterinary case report
Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR.
- Journal:
- Journal of virological methods
- Year:
- 2007
- Authors:
- Elia, Gabriella et al.
- Affiliation:
- Department of Animal Health and Well-being · Italy
- Species:
- dog
Abstract
A TaqMan real-time RT-PCR assay was developed for detection of RNA transcripts produced by replicating CPV-2. A pair of primers and a TaqMan probe targeting the spliced NS2 mRNA were designed. A synthetic DNA fragment was constructed to mimic the spliced NS2 mRNA by PCR-based gene assembly and was used for generation of standard RNAs. The detection limit of the assay was 1x10(2) RNA copies and standard curve displayed a linear range from 1x10(2) to 1x10(9) copies and a good reproducibility. The assay was then applied to determine the mRNA loads in the tissues of dogs naturally infected by CPV-2. mRNA was detected in a variety of tissues, including the central nervous system.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/17692932/