Peer-reviewed veterinary case report
Detecting invasive bacteria in dogs with granulomatous colitis using
By Ishii, Patricia Eri et al.·Published in Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·2022·Department of Small Animal Clinical Sciences, United States·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Detection of invasivein dogs with granulomatous colitis using immunohistochemistry.
- Species:
- dog
Plain-English summary
A group of dogs with granulomatous colitis, a condition that causes inflammation in the colon, was tested for an invasive bacterial infection. Researchers compared two methods for detecting this infection: the traditional fluorescence in situ hybridization (FISH) and a newer technique called immunohistochemistry (IHC). They found that IHC was just as effective as FISH in identifying the bacteria, making it a useful option for vets who may not have access to the more expensive FISH equipment. This means that IHC could help diagnose and manage dogs suffering from this type of colitis more easily.
People also search for: dog granulomatous colitis treatment · dog colitis symptoms · how to test for bacterial infection in dogs
Abstract
Granulomatous colitis in dogs can be associated with infection of the colonic mucosa by invasive strains of. To date, fluorescence in situ hybridization (FISH) is the gold-standard method to assess intramucosal and intracellular bacterial invasion. However, FISH requires expensive fluorescence microscopy equipment and is therefore not widely available. We investigated the use of immunohistochemistry (IHC) as an alternative method to detect invasivein dogs with granulomatous colitis. Archived paraffin-embedded blocks were selected from 26 dogs with colitis, in which FISH had been performed by an outside laboratory. Using a polyclonal antibody, IHC forwas performed on sections cut from the same blocks, and the presence of invasivewas recorded. All 11 specimens in which FISH had detectedwere also positive on IHC, with strong immunolabeling in the cytoplasm of macrophages and extracellularly in the lamina propria; all 15 specimens that were negative for invasive bacteria on FISH were also negative on IHC. We found that IHC is a sensitive technique for the detection of invasivein dogs with granulomatous colitis.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/35993285/