Peer-reviewed veterinary case report
How to detect mink enteritis virus in pets?
By Wang, Jianke et al.·Published in Journal of virological methods·2013·Institute of Special Economic Animal and Plant Sciences, China·View original on PubMed →
PetCaseFinder translated the abstract of this peer-reviewed paper into plain English so pet owners can read it. We do not publish original research — every detail traces back to the citation above. How we work →
Original publication title: Detection of mink enteritis virus by loop-mediated isothermal amplification (LAMP).
- Species:
- dog
Plain-English summary
Researchers have developed a new method called loop-mediated isothermal amplification (LAMP) to detect mink enteritis virus (MEV), which causes illness in minks. This method can identify the virus more quickly and sensitively than the traditional PCR test, taking just 60 minutes to complete. In their study, they tested 230 clinical samples and found that 84 were positive for MEV, which is more than the 68 positive results found using the older PCR method. This new LAMP test could be especially useful in places with limited lab resources, making it easier to diagnose MEV infections in minks. Overall, the treatment method shows promise for improving the detection of this virus.
Abstract
Loop-mediated isothermal amplification (LAMP) method was discovered in the last decade but only used for the first time in the diagnosis of mink enteritis virus (MEV) infection in this study. The amplification could be completed within 60 min, under isothermal condition at 65°C, by employing a set of four primers targeting the VP2 gene of MEV. The LAMP was more sensitive than the conventional PCR, with a detection limit of 10(-1) median tissue culture infective doses (TCID(50))/ml per reaction, compared with 10 TCID(50)/ml for PCR analysis. No cross reactivity was observed for other related viruses, including canine distemper virus (CDV) and Aleutian mink disease parvovirus (AMDV). Eighty four of 230 clinical samples were found to be positive for MEV, which is higher than that determined by using the conventional PCR method (68). The results indicate the LAMP can be potentially used to determine MEV as a simple, rapid procedure. This assay would be an available alternative to PCR analysis for the diagnosis of MEV infection in mink, particularly in less well-equipped laboratories and in rural settings where resources are limited.
Find similar cases for your pet
PetCaseFinder finds other peer-reviewed reports of pets with the same symptoms, plus a plain-English summary of what was tried across them.
Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/23183142/