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Peer-reviewed veterinary case report

Detection of rabies virus genes by in-situ polymerase chain reaction.

Journal:
Veterinary research communications
Year:
2007
Authors:
Praveena, P Ezhil et al.
Affiliation:
Indian Veterinary Research Institute

Abstract

Rabies is diagnosed by FAT in the impression smears of brain tissues. In this study, an attempt was made to diagnose rabies using in situ polymerase chain reaction (ISPCR). A digoxigenin-labelled double-stranded probe specific for a portion of the 'N' gene of rabies virus was used. Positive signals were identified as blue dots in the intraneuronal and neuropil areas.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/17260182/