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Peer-reviewed veterinary case report

Detection of Salmonella strains and Escherichia coli O157:H7 in feces of small ruminants and their isolation with various media.

Journal:
Applied and environmental microbiology
Year:
2005
Authors:
Pao, Steven et al.
Affiliation:
Virginia State University · United States

Abstract

Salmonella strains and Escherichia coli O157:H7 were detected in 17 and 5 small ruminants in Virginia, respectively, of 287 tested. Background microflora interfered with the fecal analysis. The combination of Salmonella enzyme immunoassay (EIA) detection and xylose-lysine-deoxycholate agar isolation was satisfactory. Modifying enrichment to a 1:100 dilution enabled effective E. coli O157:H7 detection by EIA and isolation by sorbitol-MacConkey agar with cefixime-tellurite.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/15812051/