Peer-reviewed veterinary case report
Detection of specific antibodies against tembusu virus in ducks by use of an E protein-based enzyme-linked immunosorbent assay.
- Journal:
- Journal of clinical microbiology
- Year:
- 2013
- Authors:
- Yin, Xiuchen et al.
- Affiliation:
- Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences · China
Abstract
We developed an enzyme-linked immunosorbent assay (ELISA) using eukaryotically expressed E protein as the antigen (termed E-ELISA) to detect antibodies to tembusu virus (TMUV) in ducks. The E-ELISA did not react with antisera to other known pathogens, indicating the E protein is specific for recognizing anti-TMUV antibodies. Compared to the serum neutralization test, the specificity and sensitivity of the E-ELISA was 93.2 and 97.8%, respectively. Therefore, this E-ELISA is a sensitive and rapid method for detecting antibodies against TMUV in ducks.
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Search related cases →Original publication: https://pubmed.ncbi.nlm.nih.gov/23616462/