Detection of thegene encoding the invasive enterohemorrhagic species0157:H7 using qPCR in horse feces samples on Sumbawa Island, Indonesia.

Abstract

BACKGROUND: Bacterial identification can be done using various testing techniques. Molecular techniques are often used to research dangerous diseases, an approach using genetic information on the pathogenic agent. The enterohemorrhagic invasive species0157:H7 was identified from the feces of working horses on the island of Sumbawa. Another advance in molecular technology is genome amplification with qPCR which is the gold standard for detecting AIM: This study aims to detect and identify the invasive species0157:H7 using the gene encodingwith the qPCR method sourced from horse feces. METHODS: Fresh fecal samples from horses on Sumbawa Island were isolated and identified, then continued with molecular examination using the gene encodingusing the qPCR method. RESULTS: qPCR testing in this study showed that six sample isolates that were positive for0157:H7 were detected for the presence of thegene, which is a gene coding for an invasive species ofbacteria. The highest to lowest Cq values and Tm from the qPCR results of the sample isolates were 15.98 (4KJ), 14.90 (19KG), 14.6 (3KJ), 13.77 (20KG), 12.56 (5KGB), and 12.20 (6KJ). Tm values are 86.7 (4KJ), 86.69 (3KJ), 86.56 (5KGB), 85.88 (20KGB), 85.81 (19KG), and 85.74 (6KJ). CONCLUSION: Validation, standardization of the development, and modification of qPCR technology must be carried out to harmonize testing throughout to avoid wrong interpretation of the test results so that the determination of actions to eradicate and control diseases originating from animals in the field does not occur.