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Peer-reviewed veterinary case report

Detection of virus particles resembling circovirus and porcine circovirus 2a (PCV2a) sequences in feces of dogs.

Journal:
Research in veterinary science
Year:
2017
Authors:
Herbst, Werner & Willems, Hermann
Affiliation:
Institute of Hygiene and Infectious Diseases of Animals · Germany
Species:
dog

Abstract

During routine electron microscopy of fecal samples from diarrheic dogs dated from 2000 virus particles resembling circovirus in shape and size were detected in two samples (V2177/00; V3374/00). Polymerase chain reaction (PCR) using primers specific for porcine circovirus type 2 (PCV2) amplified DNA recovered from both samples. Sequencing of PCR amplificates (V2177/00) obtained with PCV2-specific primer pairs revealed a genome size of 1768bp. The nucleotide sequence was highly similar (98% nucleotide identity) to the PCV2a reference sequence.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/28135670/