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Peer-reviewed veterinary case report

Detection Protocols for West Nile Virus in Mosquitoes, Birds, and Nonhuman Mammals.

Journal:
Methods in molecular biology (Clifton, N.J.)
Year:
2016
Authors:
Kauffman, Elizabeth B et al.
Affiliation:
New York State Department of Health · United States
Species:
bird

Abstract

West Nile virus is the most widespread mosquito-borne virus in the world, and the most common cause of encephalitis in the USA. Surveillance for this medially important mosquito-borne pathogen is an important part of public health practice. Here we present protocols for testing environmental samples such as mosquitoes, nonvertebrate mammals, and birds for this virus, including RT-PCR, virus isolation in cell culture, and antigenic assays, as well as serologic assays for antibody detection.

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Original publication: https://pubmed.ncbi.nlm.nih.gov/27188559/