Peer-reviewed veterinary case report
Amikacin antibiotic stability in four dog ear solutions over 56 days
By Klinczar, Arden M et al.·Published in Veterinary dermatology·2022·Animal Dermatology Clinic, United States·View original on PubMed →
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Original publication title: Determination of amikacin stability at 1% and 3% concentrations in four topical solutions over a 56-day period.
- Species:
- dog
Plain-English summary
A study looked at how stable amikacin, an antibiotic, is when mixed into different topical solutions for treating bacterial ear infections in dogs. The researchers tested four solutions over 56 days and found that amikacin remained effective in most of them, except when it was at a lower concentration in one specific solution. This means that if your vet prescribes a topical treatment with amikacin for your dog's ear infection, it should stay effective for several weeks. Always consult your veterinarian for the best treatment options for your pet's specific needs.
Abstract
BACKGROUND: Anecdotally, amikacin has been added to compounded topical preparations for the management of canine bacterial otitis externa. However, the stability of amikacin within these solutions is unknown. HYPOTHESIS/OBJECTIVES: The purpose of this study was to determine the stability of amikacin at 10 and 30 mg/mL concentrations in four topical solutions over a 56 day period. We hypothesised that amikacin would maintain chemical stability within the various solutions. METHODS AND MATERIALS: Amikacin was formulated to 10 and 30 mg/mL (1% and 3%) concentrations within four topical solutions: tris-EDTA (TrizEDTA Aqueous Flush) (TE); 0.15% chlorhexidine gluconate and tris-EDTA (TrizCHLOR Flush) (TC); 0.9% NaCl (NA); and 0.9% NaCl + 2 mg/mL dexamethasone (ND). Samples were made in duplicate and stored at room temperature (25°C) for 0, 7,14, 21, 28 and 56 days. Amikacin content was quantified, in triplicate, by ultrahigh-performance liquid chromatography tandem mass spectrometry. RESULTS: The recovered amikacin concentrations for the 10 mg/mL solutions ranged from 10 to 13.5 mg/mL (mean 11.5 mg/mL) with the exception of NA sample 2 at Day (D)0 (9.4 mg/mL) and D7 (9.2 mg/mL). The recovered amikacin concentrations for the 30 mg/mL solutions ranged from 30 to 40.2 mg/mL (mean 35.7 mg/mL). No significant difference was seen between the amikacin concentrations at D0 compared to D56 for all solutions except 10 mg/mL TE (P < 0.001). CONCLUSIONS AND CLINICAL RELEVANCE: Amikacin maintained stability within TE, TC, NA and ND over 56 days except when formulated at 10 mg/mL within TE.
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Search related cases →Original publication on PubMed: https://pubmed.ncbi.nlm.nih.gov/34545642/